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cell cycle analysis software program modfit lt version 2.0  (Verity Software House)

 
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    Verity Software House cell cycle analysis software program modfit lt version 2.0
    FoxM1 is required for <t>cell</t> <t>cycle</t> progression and survival in glioma cells with heat shock stress. A, U-87MG cells were transfected with FoxM1-specific siRNA, and 48 h after the transfection cells were treated with heat shock stress at 42 °C for the indicated times. Cell cycle <t>analysis</t> was performed with FACS. B, U-87MG cells were transfected with FoxM1-specific siRNA, and 48 h after the transfection cells were grows for the 4 days, and cell numbers were counted at each time point to generate cell growth curve. C, U-87MG cells were transfected with FoxM1-specific siRNA and 48 h after the transfection cells were treated with heat shock at 42 °C for 2 h followed by recovery for 2 h; total cell lysates were used for Western blotting. D, Hs683 cell were transfected with FoxM1 expression vector and control plasmid. 24 h after the transfection, cells were treated with heat shock stress at 42 °C for the indicated times, and cell cycle analysis was performed. E, Hs683 cells were transfected with FoxM1 expression plasmid and control vector, and 48 h after the transfection the cells were treated with heat shock at 42 °C for 2 h and recovered for 2 h. Western blotting was performed to examine the expression of FoxM1 and downstream molecules. F, U-87MG cells were transfected with FoxM1-specific siRNA, and 48 h after the transfection cells were treated with lethal heat shock stress (44 °C) for the indicated time points. Cells were stained with propidium iodide and examined under fluorescence microscope. Dead cells and apoptotic bodies were characterized by condensed or fragmented nuclei. G, U-87MG cells were transfected with FoxM1-specific siRNA. 48 h after the transfection cells were treated with lethal heat shock stress at 44 °C for different time points, viable cell numbers were counted. *, p < 0.05; **, p < 0.01.
    Cell Cycle Analysis Software Program Modfit Lt Version 2.0, supplied by Verity Software House, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/modfit+2%2E0+cell+cycle+analysis+program/cell+cycle+fitting+software+modfit/pmc03548473-74-5-14
    Average 90 stars, based on 1 article reviews
    cell cycle analysis software program modfit lt version 2.0 - by Bioz Stars, 2026-10
    90/100 stars

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    1) Product Images from "Forkhead Box M1 Is Regulated by Heat Shock Factor 1 and Promotes Glioma Cells Survival under Heat Shock Stress * "

    Article Title: Forkhead Box M1 Is Regulated by Heat Shock Factor 1 and Promotes Glioma Cells Survival under Heat Shock Stress *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M112.379362

    FoxM1 is required for cell cycle progression and survival in glioma cells with heat shock stress. A, U-87MG cells were transfected with FoxM1-specific siRNA, and 48 h after the transfection cells were treated with heat shock stress at 42 °C for the indicated times. Cell cycle analysis was performed with FACS. B, U-87MG cells were transfected with FoxM1-specific siRNA, and 48 h after the transfection cells were grows for the 4 days, and cell numbers were counted at each time point to generate cell growth curve. C, U-87MG cells were transfected with FoxM1-specific siRNA and 48 h after the transfection cells were treated with heat shock at 42 °C for 2 h followed by recovery for 2 h; total cell lysates were used for Western blotting. D, Hs683 cell were transfected with FoxM1 expression vector and control plasmid. 24 h after the transfection, cells were treated with heat shock stress at 42 °C for the indicated times, and cell cycle analysis was performed. E, Hs683 cells were transfected with FoxM1 expression plasmid and control vector, and 48 h after the transfection the cells were treated with heat shock at 42 °C for 2 h and recovered for 2 h. Western blotting was performed to examine the expression of FoxM1 and downstream molecules. F, U-87MG cells were transfected with FoxM1-specific siRNA, and 48 h after the transfection cells were treated with lethal heat shock stress (44 °C) for the indicated time points. Cells were stained with propidium iodide and examined under fluorescence microscope. Dead cells and apoptotic bodies were characterized by condensed or fragmented nuclei. G, U-87MG cells were transfected with FoxM1-specific siRNA. 48 h after the transfection cells were treated with lethal heat shock stress at 44 °C for different time points, viable cell numbers were counted. *, p < 0.05; **, p < 0.01.
    Figure Legend Snippet: FoxM1 is required for cell cycle progression and survival in glioma cells with heat shock stress. A, U-87MG cells were transfected with FoxM1-specific siRNA, and 48 h after the transfection cells were treated with heat shock stress at 42 °C for the indicated times. Cell cycle analysis was performed with FACS. B, U-87MG cells were transfected with FoxM1-specific siRNA, and 48 h after the transfection cells were grows for the 4 days, and cell numbers were counted at each time point to generate cell growth curve. C, U-87MG cells were transfected with FoxM1-specific siRNA and 48 h after the transfection cells were treated with heat shock at 42 °C for 2 h followed by recovery for 2 h; total cell lysates were used for Western blotting. D, Hs683 cell were transfected with FoxM1 expression vector and control plasmid. 24 h after the transfection, cells were treated with heat shock stress at 42 °C for the indicated times, and cell cycle analysis was performed. E, Hs683 cells were transfected with FoxM1 expression plasmid and control vector, and 48 h after the transfection the cells were treated with heat shock at 42 °C for 2 h and recovered for 2 h. Western blotting was performed to examine the expression of FoxM1 and downstream molecules. F, U-87MG cells were transfected with FoxM1-specific siRNA, and 48 h after the transfection cells were treated with lethal heat shock stress (44 °C) for the indicated time points. Cells were stained with propidium iodide and examined under fluorescence microscope. Dead cells and apoptotic bodies were characterized by condensed or fragmented nuclei. G, U-87MG cells were transfected with FoxM1-specific siRNA. 48 h after the transfection cells were treated with lethal heat shock stress at 44 °C for different time points, viable cell numbers were counted. *, p < 0.05; **, p < 0.01.

    Techniques Used: Transfection, Cell Cycle Assay, Western Blot, Expressing, Plasmid Preparation, Control, Staining, Fluorescence, Microscopy

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    Article Snippet: DNA histograms of all p53-negative and p53-positive cells were analyzed using the MODFIT 2.0 cell cycle analysis program (Verity Software, Topersham, MA).



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    FoxM1 is required for <t>cell</t> <t>cycle</t> progression and survival in glioma cells with heat shock stress. A, U-87MG cells were transfected with FoxM1-specific siRNA, and 48 h after the transfection cells were treated with heat shock stress at 42 °C for the indicated times. Cell cycle <t>analysis</t> was performed with FACS. B, U-87MG cells were transfected with FoxM1-specific siRNA, and 48 h after the transfection cells were grows for the 4 days, and cell numbers were counted at each time point to generate cell growth curve. C, U-87MG cells were transfected with FoxM1-specific siRNA and 48 h after the transfection cells were treated with heat shock at 42 °C for 2 h followed by recovery for 2 h; total cell lysates were used for Western blotting. D, Hs683 cell were transfected with FoxM1 expression vector and control plasmid. 24 h after the transfection, cells were treated with heat shock stress at 42 °C for the indicated times, and cell cycle analysis was performed. E, Hs683 cells were transfected with FoxM1 expression plasmid and control vector, and 48 h after the transfection the cells were treated with heat shock at 42 °C for 2 h and recovered for 2 h. Western blotting was performed to examine the expression of FoxM1 and downstream molecules. F, U-87MG cells were transfected with FoxM1-specific siRNA, and 48 h after the transfection cells were treated with lethal heat shock stress (44 °C) for the indicated time points. Cells were stained with propidium iodide and examined under fluorescence microscope. Dead cells and apoptotic bodies were characterized by condensed or fragmented nuclei. G, U-87MG cells were transfected with FoxM1-specific siRNA. 48 h after the transfection cells were treated with lethal heat shock stress at 44 °C for different time points, viable cell numbers were counted. *, p < 0.05; **, p < 0.01.
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    FoxM1 is required for <t>cell</t> <t>cycle</t> progression and survival in glioma cells with heat shock stress. A, U-87MG cells were transfected with FoxM1-specific siRNA, and 48 h after the transfection cells were treated with heat shock stress at 42 °C for the indicated times. Cell cycle <t>analysis</t> was performed with FACS. B, U-87MG cells were transfected with FoxM1-specific siRNA, and 48 h after the transfection cells were grows for the 4 days, and cell numbers were counted at each time point to generate cell growth curve. C, U-87MG cells were transfected with FoxM1-specific siRNA and 48 h after the transfection cells were treated with heat shock at 42 °C for 2 h followed by recovery for 2 h; total cell lysates were used for Western blotting. D, Hs683 cell were transfected with FoxM1 expression vector and control plasmid. 24 h after the transfection, cells were treated with heat shock stress at 42 °C for the indicated times, and cell cycle analysis was performed. E, Hs683 cells were transfected with FoxM1 expression plasmid and control vector, and 48 h after the transfection the cells were treated with heat shock at 42 °C for 2 h and recovered for 2 h. Western blotting was performed to examine the expression of FoxM1 and downstream molecules. F, U-87MG cells were transfected with FoxM1-specific siRNA, and 48 h after the transfection cells were treated with lethal heat shock stress (44 °C) for the indicated time points. Cells were stained with propidium iodide and examined under fluorescence microscope. Dead cells and apoptotic bodies were characterized by condensed or fragmented nuclei. G, U-87MG cells were transfected with FoxM1-specific siRNA. 48 h after the transfection cells were treated with lethal heat shock stress at 44 °C for different time points, viable cell numbers were counted. *, p < 0.05; **, p < 0.01.
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    FoxM1 is required for <t>cell</t> <t>cycle</t> progression and survival in glioma cells with heat shock stress. A, U-87MG cells were transfected with FoxM1-specific siRNA, and 48 h after the transfection cells were treated with heat shock stress at 42 °C for the indicated times. Cell cycle <t>analysis</t> was performed with FACS. B, U-87MG cells were transfected with FoxM1-specific siRNA, and 48 h after the transfection cells were grows for the 4 days, and cell numbers were counted at each time point to generate cell growth curve. C, U-87MG cells were transfected with FoxM1-specific siRNA and 48 h after the transfection cells were treated with heat shock at 42 °C for 2 h followed by recovery for 2 h; total cell lysates were used for Western blotting. D, Hs683 cell were transfected with FoxM1 expression vector and control plasmid. 24 h after the transfection, cells were treated with heat shock stress at 42 °C for the indicated times, and cell cycle analysis was performed. E, Hs683 cells were transfected with FoxM1 expression plasmid and control vector, and 48 h after the transfection the cells were treated with heat shock at 42 °C for 2 h and recovered for 2 h. Western blotting was performed to examine the expression of FoxM1 and downstream molecules. F, U-87MG cells were transfected with FoxM1-specific siRNA, and 48 h after the transfection cells were treated with lethal heat shock stress (44 °C) for the indicated time points. Cells were stained with propidium iodide and examined under fluorescence microscope. Dead cells and apoptotic bodies were characterized by condensed or fragmented nuclei. G, U-87MG cells were transfected with FoxM1-specific siRNA. 48 h after the transfection cells were treated with lethal heat shock stress at 44 °C for different time points, viable cell numbers were counted. *, p < 0.05; **, p < 0.01.
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    FoxM1 is required for cell cycle progression and survival in glioma cells with heat shock stress. A, U-87MG cells were transfected with FoxM1-specific siRNA, and 48 h after the transfection cells were treated with heat shock stress at 42 °C for the indicated times. Cell cycle analysis was performed with FACS. B, U-87MG cells were transfected with FoxM1-specific siRNA, and 48 h after the transfection cells were grows for the 4 days, and cell numbers were counted at each time point to generate cell growth curve. C, U-87MG cells were transfected with FoxM1-specific siRNA and 48 h after the transfection cells were treated with heat shock at 42 °C for 2 h followed by recovery for 2 h; total cell lysates were used for Western blotting. D, Hs683 cell were transfected with FoxM1 expression vector and control plasmid. 24 h after the transfection, cells were treated with heat shock stress at 42 °C for the indicated times, and cell cycle analysis was performed. E, Hs683 cells were transfected with FoxM1 expression plasmid and control vector, and 48 h after the transfection the cells were treated with heat shock at 42 °C for 2 h and recovered for 2 h. Western blotting was performed to examine the expression of FoxM1 and downstream molecules. F, U-87MG cells were transfected with FoxM1-specific siRNA, and 48 h after the transfection cells were treated with lethal heat shock stress (44 °C) for the indicated time points. Cells were stained with propidium iodide and examined under fluorescence microscope. Dead cells and apoptotic bodies were characterized by condensed or fragmented nuclei. G, U-87MG cells were transfected with FoxM1-specific siRNA. 48 h after the transfection cells were treated with lethal heat shock stress at 44 °C for different time points, viable cell numbers were counted. *, p < 0.05; **, p < 0.01.

    Journal: The Journal of Biological Chemistry

    Article Title: Forkhead Box M1 Is Regulated by Heat Shock Factor 1 and Promotes Glioma Cells Survival under Heat Shock Stress *

    doi: 10.1074/jbc.M112.379362

    Figure Lengend Snippet: FoxM1 is required for cell cycle progression and survival in glioma cells with heat shock stress. A, U-87MG cells were transfected with FoxM1-specific siRNA, and 48 h after the transfection cells were treated with heat shock stress at 42 °C for the indicated times. Cell cycle analysis was performed with FACS. B, U-87MG cells were transfected with FoxM1-specific siRNA, and 48 h after the transfection cells were grows for the 4 days, and cell numbers were counted at each time point to generate cell growth curve. C, U-87MG cells were transfected with FoxM1-specific siRNA and 48 h after the transfection cells were treated with heat shock at 42 °C for 2 h followed by recovery for 2 h; total cell lysates were used for Western blotting. D, Hs683 cell were transfected with FoxM1 expression vector and control plasmid. 24 h after the transfection, cells were treated with heat shock stress at 42 °C for the indicated times, and cell cycle analysis was performed. E, Hs683 cells were transfected with FoxM1 expression plasmid and control vector, and 48 h after the transfection the cells were treated with heat shock at 42 °C for 2 h and recovered for 2 h. Western blotting was performed to examine the expression of FoxM1 and downstream molecules. F, U-87MG cells were transfected with FoxM1-specific siRNA, and 48 h after the transfection cells were treated with lethal heat shock stress (44 °C) for the indicated time points. Cells were stained with propidium iodide and examined under fluorescence microscope. Dead cells and apoptotic bodies were characterized by condensed or fragmented nuclei. G, U-87MG cells were transfected with FoxM1-specific siRNA. 48 h after the transfection cells were treated with lethal heat shock stress at 44 °C for different time points, viable cell numbers were counted. *, p < 0.05; **, p < 0.01.

    Article Snippet: Data were analyzed with a cell cycle analysis software program (ModFit LT Version 2.0; Verity Software House) to calculate the percentage of cells at G 1 , S, and G 2 phase.

    Techniques: Transfection, Cell Cycle Assay, Western Blot, Expressing, Plasmid Preparation, Control, Staining, Fluorescence, Microscopy